I have to see Japan before I die, that's a definite must.
I would also love to go to Englannd and visit the Hundred Acre Wood, because I adore Winnie the Pooh and it would be so amazing to visit the Enchanted Place and see the memorial to A.A. Milne there.
I'm also an artist and I love DaVinci, so I'd love to visit the Louvre and see the Mona Lisa along with DaVinci's other beautiful works. I'd also love to see the Sistine Chapel, the artwork all over that city is stunning.
I'm also a big theatre geek, so I'd love to go to New York and see a show on Broadway. I saw the Lion King broadway show once when it was on tour and it was incredible! I'd love to see it again.
My boyfriend and I would also love to go to Otakon together one day, it's the 2nd largest anime convention in the states. If at all possible, we'd probably kill to go to Jump Fest, it's an anime convention held in Japan and it's the largest anime convention in the world.
There are more places, but those are the top places I want to see before I die.