Well I really need help on a lab in Biology. I don't really know what we are trying to find or do. Basically we are supposed to put "a single colony of bacteria" into two micro test tube (one labeled +DNA and one labeled -DNA) and then put in some plasmid "solution" into the test tube labeled +DNA only.And then we put it in ice, then in a water bath, then back to ice, etc (i think this part isn't that important). We then put it in room temperature for 10 minutes and then we use a pipet and put the solution into the "plates" labeled LB (thats the food for the bacteria to grow in he said), amp (don't know what this is, and ara (he said it was used to "turn on" something I forgot what it was).
I don't know if any of this makes sense, I have the procedure right next to me and we are going to do the lab and he told use to KNOW what we were doing and to not just follow the procedures. If you want to know one of the steps or if something is not clarified just tell me, I really want to know what we are doing!